Adipocyte rna-seq

Author: wzbp

August undefined, 2024

WebMar 27, 2024 · The KEGG metabolic pathway suggested a potential role for arachidonic acid metabolism, serotonergic synapses, neuroactive ligand-receptor interactions, tyrosine metabolism, and regiomelanin. Vitamin digestion and absorption, as well as regulation of lipolysis in adipocytes, were the main metabolic pathways that distinguished the T vs. M … WebMar 8, 2024 · We performed RNA-Seq on adipocytes isolated from three different adipose depots (BAT, iWAT, and eWAT) in HFD and ND. More than 200 million paired-end reads …

A Transcriptomic Analysis Reveals Novel Patterns of Gene Expression …

WebOct 23, 2024 · Additionally, single-cell RNA-sequencing (scRNA-Seq) of adipose stromal populations revealed an increase in IL10-producing adaptive immune cells under … WebSep 16, 2024 · The transcriptome data was generated from the different stages of the 3T3-L1 adipocytes using RNA-seq technology. Table 2 briefly summarizes the information of … show variables like char%

Single-cell transcriptional networks in differentiating

WebFeb 8, 2024 · RNA-seq was used to determine genes regulated by EGCG and identify the key differences between the two functionally distinct adipose tissues. Taken … WebDec 3, 2024 · Transcriptome-wide m 6 A-Seq Reveals Global m 6 A Modification Patterns During Yak Adipocyte Differentiation. The yak adipocyte and preadipocyte of three biological replicates were used for transcriptome-wide m 6 A-sequencing (m 6 A-seq) and RNA-sequencing (RNA-seq) assays. In total, 12 libraries were sequenced, comprising … WebApr 30, 2024 · Single-cell RNA-seq profiles of human preadipocytes during adipogenesis in vitro identifies at least two distinct classes of subcutaneous white adipocytes. These … show variables like innodb_log_file_size

Genes Free Full-Text Transcriptome Profiling of Different ...

A single-cell atlas of human and mouse white adipose

WebJul 11, 2024 · RNA sequencing followed by whole genome–wide expression analysis shows that beige adipocytes induced from bat aWAT, rather than sWAT, have molecular signatures resembling those of mouse sWAT-induced beige adipocytes and exhibit dynamic profiles similar to those of classical brown adipocytes. WebApr 11, 2024 · Background: Insulin resistance (IR) is a major contributing factor to the pathogenesis of metabolic syndrome and type 2 diabetes mellitus (T2D). Adipocyte metabolism is known to play a crucial role in IR. Therefore, the aims of this study were to identify metabolism-related proteins that could be used as potential biomarkers of IR and … show variables like max_connectionsWebWhite adipose tissue (WAT) distribution is sex dependent. Adipocyte hyperplasia contributes to WAT distribution in mice driven by cues in the tissue m… show variables like port

"WebSeveral studies have highlighted the relevance of lncRNAs in adipocyte differentiation, but the precise molecular mechanism has largely remained elusive. In the present study, we … " - Adipocyte rna-seq

Adipocyte rna-seq

Novel insights into adipose tissue heterogeneity SpringerLink

WebJun 1, 2024 · RNA-seq was employed to acquire the pectorales and crureuse transcriptome of ZD and AA chickens, with the aim of determining the potential molecular mechanism underlying chicken meat quality, flavor, and growth and development. We selected four mix sample separate cDNA libraries for RNA-seq. WebMar 16, 2024 · As expected, adipocytes were found only in the sNuc-seq dataset. The sNuc-seq data were also enriched for vascular cells and macrophages, probably because collagenase digestion did not fully...

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WebNational Center for Biotechnology Information WebSep 22, 2024 · In COVID-19 autopsy cases, we identified SARS-CoV-2 RNA in adipocytes with an associated inflammatory infiltrate. We identified two distinct cellular targets of infection: adipocytes and a subset of inflammatory adipose tissue–resident macrophages.

WebSep 9, 2016 · Adipogenesis of primary brown preadipocytes In order to get a global view of gene expression during adipogenesis of brown preadipocytes, RNA-Seq was performed to identify the genes involved and their function. RNA was isolated from primary cultured brown preadipocytes (D0) and differentiated adipocytes (D7). WebNov 27, 2024 · Single‐cell RNA sequencing and lipidomics reveal cell and lipid dynamics of fat infiltration in skeletal muscle - PMC Back to Top Skip to main content An official website of the United States government Here's how you know The .gov means it’s official. Federal government websites often end in .gov or .mil.

WebDec 21, 2024 · Single nucleus sequencing (snRNAseq) is a modified version of scRNAseq, wherein a single nucleus is sequenced for actively transcribed RNA instead of a single cell. This technique is especially effective on tissues that cannot be dissociated into intact single cells, such as adipocytes. WebNov 7, 2024 · RNA was prepared from eight different cell WAT cell types (ASCs, total adipose tissue macrophages (ATMs), M1 ATMs, M2 ATMs, total T cells, CD4 + T cells, CD8 + T cells, and mature adipocytes). Ten nanograms of RNA was amplified using four cycles and loaded onto Clariom™D microarray chips. For details see Additional file 1: …

WebG protein-coupled receptors (GPCRs) are expressed essentially on all cells, facilitating cellular responses to external stimuli, and are involved in nearly every biological process. Several members of this family play significant roles in the regulation of adipogenesis and adipose metabolism. However, the expression and functional significance of a vast …

WebIn addition, adipose tissue functions as a signalling hub that regulates systemic metabolism through paracrine and endocrine signals 4. Here we use single-nucleus RNA-sequencing (snRNA-seq) analysis in mice and humans to characterize adipocyte heterogeneity. show variables like secure_authWebThese RNA sequencing data involved macrophage polarization, which was important for macrophage inflammation and energy metabolism in adipose tissues. First, we found 768 genetic markers of M1 macrophages from the published bulk RNA dataset GSE69607 that showed higher expression levels in M1 macrophages than other types. show variables like local_infileWebJul 5, 2024 · Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth of obesity and metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meti … show variables like timeoutWebJan 3, 2024 · To identify smORF-containing transcripts that are adipose specific, we analyzed mouse tissue RNA sequencing (RNA-seq) data from the ENCODE mouse project. 45 We then selected the smORF-containing transcripts with a TPM value of at least 1 and included these as a subset in an UpSet plot, only retaining groups with at least 20 … show variables like wait_timeoutWebAug 8, 2024 · RNA extraction and RNA-seq analysis were performed by NovoGene (Beijing, China), the sequencing platform was illumina novaseq 6000, and the read length was 150bp. The sampling time points were listed in Figure 1. FeatureCounts (1.5.0-p3) is used to calculate the reads mapped to each gene. show variables like secureWebMar 25, 2024 · However, single-nuclei RNA sequencing (sn-RNAseq) and spatial transcriptomics have created an avenue to explore the complexity of adipocyte status and function in vivo. While current knowledge keeps expanding, it is now clear that distinct adipocyte populations exist within WAT depots. show variables like slow_query_logWebOct 28, 2024 · To comprehensively characterize mature subpopulations of brown adipocytes, we performed single-nucleus RNA sequencing (snRNA-seq) 12, 13 in … show variety of gifts at coles bookstore