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Web10 de ago. de 2024 · Each sample was prepared in duplicate in a volume of 20 μL containing 10 μL of Hieff UNICON ® qPCR SYBR Green Master Mix (Cat No. 11198, Yeasen), 0.3 μL of each primer (10 ppm), 2 μL of genomic DNA, and 7.4 μL of ddH 2 O. WebProduct Description. Hieff Unicon TM TaqMan Multiplex qPCR Master Mix (UDG plus) is a fluorescent quantitative premix that uses the company's next-generation antibody …
Hieff unicon
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Web30 de set. de 2024 · Ouvir: Uso do hífen: 18 regras para entender como empregar o hífen 0:00. O hífen é um sinal ortográfico com o formato de um pequeno traço horizontal ( - ). … WebProduct Description. Hieff Unicon TM Hotstart Direct Taq DNA Polymerase is a "hot start" DNA polymerase that tolerates blood and other repressors. This product is blocked by an …
WebHieff Unicon™ Hotstart Direct Taq DNA Polymerase, 5 U/μL. Hieff UNICON™ Hotstart J-Taq DNA polymerase, 5 U/μL. Hieff Unicon™ Universal Multiplex qPCR Master Mix … Web1 de mai. de 2024 · The qPCR was performed using Hieff Unicon® qPCR SYBR Green Master Mix (Yeasen Biotech Co., Ltd.) on CFX Connect (Bio-Rad) under conditions as follow: 95 °C, 3 min; 40 cycles of [95 °C, 5 s; 60 °C, 30 s]. Primers used are listed in Table 2.
Web19 de jul. de 2024 · Fe (II) specifically accumulated in inflammatory-activated macrophages. THP-1 cells were differentiated into M0 macrophages through PMA stimulation and resting. The M0 cells were polarized to M1, M2a, M2b, M2c, and M2d cells by various stimulation methods. COU-LIP-1 was added to the cells at 10 μM to measure intracellular Fe (II) … Web1 de out. de 2024 · Total RNA of liver tissue was isolated using Trizol as previously described [28].Total RNA was transcribed using the Hifair® II 1st Strand cDNA Synthesis Kit to synthesize cDNA. cDNA samples were used for real-time PCR in a total volume of 20 μL using Hieff UNICON® Power qPCR SYBR Green Master Mix (Yeasen, China) and …
Web31 de jan. de 2024 · RT–PCR primers were set on exons adjacent to the processed intron to determine whether any alternative variants were produced. qPCR was performed to check whether there were any significant differences in expression in edited cells. qPCRs were processed using Hieff UNICON Universal Blue qPCR SYBR Green Master Mix (Yeasen) … dyson replacement head v10Web(13086ES) Hieff Unicon® Hotstart J-Taq DNA polymerase 抗体法热启动J-Taq DNA聚合酶. 2024-04-14 (11211ES) Hieff Unicon® Universal TaqMan multiplex qPCR master mix. 2024-04-14 (12211ES) Hieff NGS® Methyl-seq DNA library Prep kit for Illumina 甲基化DNA ... dyson replacement battery svo4Web30 de nov. de 2024 · The related primer sequences are shown in Table S1. qRT-PCR was conducted following the instructions for the Hieff UNICON® qPCR SYBR® Green Master Mix (Yeasen, Shanghai, China). The amplification system was 20 μL: Hieff UNICON® qPCR SYBR® Green Master Mix 10 μL, cDNA 1 μL, upstream and downstream primer 0.8 μL … dyson replacement head animalWeb本产品是2×Mix预混合试剂，能够实现在单个反应孔中进行多重荧光定量PCR。本产品含有基因改造的热启动Taq酶，极大地提高了扩增灵敏度和特异性，本产品对多重反应缓冲体系 … csec idfWebHieff UNICON® Universal Blue qPCR SYBR Master Mix（男神版） 市场价 ￥1,136.00 好评率 100% (0)条评论 商城价 登录后可查看价格 dyson replacement filter v10 animalWeb24 de fev. de 2024 · Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the Hieff Unicon qPCR SYBR Green Master Mix (Yeasen Biotechnology, Shanghai, China) on an Applied Biosystems … dyson replacement filters targethttp://hyphenreader.com/ dyson replacement filters for air fans