High-throughput screens for eef-2 kinase

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WebMay 1, 2014 · eEF-2 kinase dictates cross-talk between autophagy and apoptosis induced by Akt Inhibition, thereby modulating cytotoxicity of novel Akt inhibitor MK-2206. ... High-throughput screens for eEF-2 kinase. J Biomol Screen, 19 (2013), pp. 445-452. Google Scholar. Diggle et al., 1998. WebOct 26, 2015 · High-Throughput Screens for eEF-2 Kinase. Article. Sep 2013; Ramakrishna Edupuganti; Ashwini K Devkota; Mangalika Warthaka; Kevin Dalby; eEF-2 kinase is a potential therapeutic target for breast ...

Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for ...

WebJul 13, 2024 · In the same study, the identification of a selective but weak inhibitor of eEF2K (IC 50 = 0.28 μM) called A-484954 through a high-throughput screen was also reported … WebJan 24, 2014 · Using this high-throughput assay, we screened more than 7000 compounds and discovered additional small molecules that inhibit the ATM kinase and further validated these hits by secondary assays. Introduction irctc nse

Eukaryotic elongation factor 2 kinase as a drug target in cancer, …

WebHere, we describe the purification of the protein kinase, eEF-2K, and the utilization of three biochemical assays in the course of identifying small molecules that block its enzymatic … WebSep 27, 2013 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … WebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput … order duac online

eEF2K: an atypical kinase target for cancer

Kinase assays BMG LABTECH

WebMay 21, 2016 · Eukaryotic elongation factor 2 kinase (eEF2K) inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was … WebMay 20, 2024 · Background: Eukaryotic elongation factor 2 kinase (eEF2K) regulates the elongation stage of protein synthesis by phosphorylating eEF2, a process related to various diseases including cancer and cardiovascular and neurodegenerative diseases. In this study, we describe the identification of novel eEF2K inhibitors using high-throughput screening … order duck for christmasWebNov 26, 2007 · Kinases are important drug discovery targets for a wide variety of therapeutic indications; consequently, the measurement of kinase activity remains a common high-throughput screening (HTS) application. Recently, enzyme-coupled luciferase-kinase (LK) format assays have been introduced. irctc number

"WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput assay systems may expedite the process. Two high-throughput assays are described for eEF-2K using recombinant, tag-free enzyme purified from bacteria. " - High-throughput screens for eef-2 kinase

High-throughput screens for eef-2 kinase

Eukaryotic elongation factor 2 kinase as a drug target in

WebHigh-throughput screens for eEF-2 kinase (Q35002039) From Wikidata. Jump to navigation Jump to search. scientific article. edit. Language Label Description Also known as; English: High-throughput screens for eEF-2 kinase. scientific article. Statements. instance of. scholarly article. 1 reference. stated in. WebMay 21, 2016 · Search worldwide, life-sciences literature Search. Advanced Search

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WebJul 15, 2014 · Eukaryotic elongation factor-2 kinase (eEF2K), encoded by the EEF2K gene, is well-known to be a Ca 2+ /calmodulin (CaM)-dependent kinase which can negatively modulate protein synthesis. It is highly conserved among eukaryotes from mammals to invertebrates, of which human and mouse may have 99 % overall amino acid identity. WebJun 10, 2014 · High-throughput screening platforms have proved beneficial for protein kinase drug discovery and development, as large compound libraries can be screened with ease and compound potency and selectivity across a subset of the kinome can be determined [13], [14].

WebSep 18, 2024 · A high-throughput screen (kinase reaction performed at room temperature) revealed the compound TCMDS-135051 as a highly selective and specific Pf CLK3 kinase inhibitor. This was confirmed in vivo by the finding that parasite strains that developed resistance against TCMDS-135051 acquired mutations in Pf CLK3. WebNov 1, 2016 · Purified human eEF2K was obtained from an expression system and a luminescence-based high-throughput screening (HTS) assay was developed using MH-1 …

WebAbstract. eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of …

WebMay 1, 2014 · The activity of eEF-2, the main eEF involved in the translocation step of elongation, can be inhibited by the eukaryotic elongation factor 2 kinase (eEF2K) (Ma, 2024). eEF2K is an...

WebAug 24, 2024 · The High-throughput screening method screens millions of chemical and biological compounds in a short interval of time. It is an automated process and screens many biological or chemical compounds for their therapeutic potential. ... As an example, the high-throughput screening process successfully identified a potent pan-SRC kinase … irctc offer for saleWebFeb 14, 2024 · To identify kinases that would regulate brown adipocyte differentiation and formation, we developed a high-content and high-throughput in vitro image-based screening system that combined two experimental approaches (fig. S1A). order ducks onlineWebSep 1, 2024 · If you are considering a high throughput screen for kinase inhibitors you should really look no further than the PHERAstar. It measures two emission wavelengths at the same time. This is required for the detection modes such as fluorescence polarization (FP) and TR-FRET, which are important in measuring kinases. order duck breastWebApplication of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening Authors: Clint D. … irctc number of tickets per monthWebeEF-2 kinase is a highly conserved protein kinase in the calmodulin -mediated signaling pathway that links multiple up-stream signals to the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 ( EEF2) and thus inhibits the EEF2 function. [6] [8] Activation [ edit] order duff cakeWebWe report here the development and optimization of a simple 384-well colorimetric assay to measure H(2)O(2) generated by the redox cycling of compounds incubated with reducing agents in high-throughput screening (HTS) assay buffers. The phenol red-horseradish peroxidase (HRP) assay readily detected H(2)O(2) either added exogenously or generated … irctc officeWebThese yeast-based methods range from HTP screens to identify novel hits against promising parasite kinase targets to the identification of potential antiparasitic kinase inhibitors extracted from databases of yeast chemical genetic screens. Keywords: Drug discovery; Drug screening; High-throughput; Kinase inhibitors; Neglected diseases; Yeast. irctc nse share price today