Mouse atf4
NettetLevels of ATF4 are decreased in inflamed intestinal mucosa from patients with active CD or UC. In mice, ATF4 deficiency reduces glutamine uptake by intestinal epithelial cells … Nettet10835-1-AP targets ATF4 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Mouse atf4
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NettetWe hypothesized that increased activity of ATF4, the mammalian equivalent of yeast GCN4, might be characteristic of mutations that extend mouse life span. Fibroblasts from the skin of two such mutants (Snell dwarf and PAPP-A knockout) were found to have higher levels of ATF4 protein and expression of several ATF4 target genes in … NettetThese antibodies target ATF4 in Human, Rat, Mouse and Zebrafish samples. Our ATF4 polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Cell treatment, Knockout and Relative expression to confirm specificity to ATF4. Find the ATF4 antibody that fits your …
NettetMouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies. Confocal … NettetAtf4 tm1Tow: Name: activating transcription factor 4; targeted mutation 1, Timothy Townes: MGI ID: MGI:2180373: Synonyms: Atf4-, Atf4 KO, Atf4 tm1Tmt: Gene: Atf4 …
NettetTXLNG overexpression significantly improved IR induced by HFD in mouse models by reducing body weight and epididymal adipose weight, decreasing mRNA expression ... had no effect on ATF4 protein levels, while ATF4 overexpression increased ATF4 protein levels. Furthermore, ATF4 overexpression notably abolished the improvements in IR … NettetMonoclonal Antibody for studying ATF-4. Cited in 3 publications. Validated for Western Blotting, Immunofluorescence (Immunocytochemistry). Highly specific and rigorously …
Nettet7. apr. 2024 · The mouse model is therefore a useful tool for imaging ATF4 translational activation at various levels, from cell cultures to whole bodies, and it has a range of …
Nettet26. jul. 2004 · Here we describe the mechanism regulating ATF4 expression involving the differential contribution of two upstream ORFs (uORFs) in the 5′ leader of the mouse ATF4 mRNA. The 5′ proximal uORF1 is a positive-acting element that facilitates ribosome scanning and reinitiation at downstream coding regions in the ATF4 mRNA. midtown psychological servicesNettet28. mar. 2024 · ATF4 and NRF2 coordinately regulate Slc7a11 expression (A) Schematic representation of the mouse Slc7a11 gene promoter with the upstream ARE and the two AAREs (AARE-1 and AARE-2) indicated by open and solid rectangles, respectively, fused to a luciferase reporter (B-E) Luciferase assay measuring Slc7a11 promoter activity … new technology in solar power generationNettet19. nov. 2015 · (D) Percentage of input of the ATF4 antibody using mouse FL cells based on the chromatin immunoprecipitation (ChIP) assay. Primers were designed to amplify … midtown pta madison alNettet1. mar. 2024 · When building healthy tissue, the human body must carefully control the growth of new cells to prevent them from becoming cancerous. A core component of this regulation is the protein mTORC1, which responds to various growth-stimulating factors and nutrients, and activates the chemical reactions cells need to grow. Part of this … new technology in testingNettet7. apr. 2016 · Using various knockout cells, we revealed that the increase in IRE1α expression depended on PERK-ATF4 pathway. PERK knockout decreased the ratio of XBP1 mRNA splicing. These findings indicated ... mid town pub 2405 allen blvd middletonNettetDescription. Atf4 is located on chromosome 15 of mice. CRISPR/Cas9 technology is used to design sgRNA and ssDNA. Through high-throughput electrotransformation of fertilized eggs, conditional knockout mice of Atf4 gene are obtained. After sexual maturity, sperms are taken and frozen. midtown psychiatry las vegas nvNettet18. aug. 2016 · The PERK pathway is the most important and also includes the phosphorylation of eukaryotic translation initiation factor 2α (eIF2α) and ATF4. In this … midtown public health office