Pef6/myc-his

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August undefined, 2024

WebpEF6/myc-His A Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … WebpcDNA-HA and pEF6-MYC:His 6 (Invitrogen). Oligo- ... Basel, Switzerland) or anti-MYC antibodies (mouse IgG1, clone 9E10, SCB, Santa Cruz Biotechnology, CA, US) for 2 hours at 4°C with agitation ...

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WebApr 27, 2007 · The pEF6- lacZ expression construct was obtained from Invitrogen. Cell Culture and Transfection —DT40 chicken B cells were grown in RPMI supplemented with 10% fetal bovine serum, 1% chicken serum, 100 units/ml penicillin, 100 μg/ml streptomycin, 50 μ m mercaptoethanol, and 20 m m GlutaMAX. http://www.labbase.net/Supply/SupplyItems-4229347.html fat pats on westmark

pEF6/Myc-HisC (VET1244) - Creative Biogene

WebJun 1, 2016 · pEF6/Myc-His vector harboring C-terminally FLAG-tagged human PLA/AT-2 cDNA [ 9] ( see Note 1 ). 7. Branson Sonifier model 250 equipped with a tapered microtip (end diameter of 1/8 in.) (Branson, Danbury, CT, USA). 8. Buffer C: 20 mM Tris–HCl (pH 7.4). 9. Buffer D: 50 mM Tris–HCl (pH 7.4), 150 mM NaCl, 0.05 % (w/v) Nonidet P-40. 10. WebpEF6 V5-His A Mammalian vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins. For other reading frames, use pEF6/V5-His B or pEF6/V5-His … WebMar 9, 2015 · pEF4/myc-His vectors are shipped on wet ice. Upon receipt, store vectors at -20°C. Kit Contents All vectors are supplied as detailed below. Store the vectors at –20°C. … friday the 13th betsy palmer

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pEF4/myc-His A, B, and C - Thermo Fisher Scientific

WebMar 3, 2024 · A reciprocal experiment was also conducted with XPF (311–469)-Myc-His 6 as well as XPF (Δ719–916)-Myc-His 6 containing the 311–469 region but lacking C-terminal HhH 2 domain (Figure 5b). After immunoprecipitation with anti-Flag antibody, weak signals of both proteins were detected, although co-IP efficiency of XPF (Δ719–916) is much ... WebThe total amount of DNA was adjusted with empty vector pEF6/Myc-His. After 48 h, cell lysates were prepared and immunoprecipitated by anti-FLAG antibody resin. Each sample … friday the 13th bed sheetsWebDec 4, 2024 · The pEF6/Myc-His vectors harbouring FL-cPLA 2 ɛ and its mutants or insert-free pEF6/Myc-His vector were introduced into HEK293 cells using Lipofectamine 2000. After 48 h of transfection, cells were harvested and sonicated twice each for 5 s in 20 mM Tris–HCl (pH 7.4). fat pat the water rat song

"WebAug 14, 2015 · zt283 pEF1/myc-His B 载体 Invitrogen 哺乳动物表达载体系统. zt284 pcDNA3.1(+)/Myc HisB 载体 Invitrogen 哺乳动物表达载体系统. zt285 pEF6/Myc-HisC 载体 Invitrogen 哺乳动物表达载体系统. zt286 pCMV-Tag 2 B 载体 Invitrogen 哺乳动物表达载体系 … " - Pef6/myc-his

Pef6/myc-his

WebSep 25, 2024 · pEF6/Myc-His vector harboring C-terminally FLAG-tagged human PLAAT-2 cDNA [ 12] ( see Note 1 ). 8. Branson Sonifier model 250 equipped with a tapered microtip (end diameter of 1/8 inch) (Branson, Danbury, CT, USA). 9. Buffer C: 20 mM Tris-HCl (pH 7.4). 10. Buffer D: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.05% (w/v) Nonidet P-40. 11. WebMar 3, 2024 · (a and b) Myc-His 6-tagged version of wild-type or truncated XPF was transiently expressed with Flag-ERCC1 in XP2YO(SV) cells. Cell lysates were …

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WebMar 5, 2007 · The pEF6- lacZ expression construct was obtained from Invitrogen. Cell culture and transfection DT40 chicken B cells were grown in RPMI supplemented with 10% foetal bovine serum, 1% chicken serum, 100 units/ml penicillin, 100 μg/ml streptomycin, 50 μM mercaptoethanol and 20mM GlutaMAX. WebpIRESneo, pcDNA3.1/Zeo, pEF6/Myc-His A and pZeoSV2 expression vectors. The resistance genes for selection of stable cell lines in these vectors were puromycin, neomycin phosphotrans-ferase, zeocin, blasticidin and zeocin, respectively. Cell culture and transfections CHO-K1, HEK 293, NIH-3T3 and HeLa cells were propagated in DMEM …

WebNov 1, 2010 · A series of protein expression vectors were constructed based on the pEF6/myc-His vector. To generate the pEF6/myc-His vector, a pSecTag2 vector (Invitrogen, USA) was digested with NotI and PmaCI, and the resulting 1.7 kb fragment was inserted into the 4.1 kb NotI – PmaCI digested pEF6/V5-His vector. WebThe vector pEF6-myc-HisB, which has an EF promotor, was chosen because of its ability to drive strong expression of the protein of interest in mammalian cells. The readers should …

WebObjectives: To determine whether PNP is associated with autoreactivity against the armadillo-repeat-containing plakophilin-3 (PKP3) protein. Methods: HEK293 cells were transiently transfected with either a pEF6/myc-His or a pEGFP-N2 construct, both encoding human PKP3 (protein products of 85 kDa and 115 kDa, respectively). WebThe total amount of DNA was adjusted with empty vector pEF6/Myc-His. After 48 h, cell lysates were prepared and immunoprecipitated by anti-FLAG antibody resin. Each sample was analyzed by western blotting with the indicated antibodies. Techniques Used: Immunoprecipitation, Expressing, Transfection, Plasmid Preparation, Western Blot

WebExpression plasmids for Myc/His-tagged WT and C329S HRD1 were constructed as described previously (Chiba Institute of Science, Japan)(Kaneko et al., 2002). ... (Chen et al., 2006). WT and R2M gp78 cDNA were inserted into the KpnI and XbaI sites of pEF6/Myc-His vector (Invitrogen). Expression plasmids for Flag-tagged WT and C42S RMA1 were ...

WebComments for pEF6/Myc-His A 5798 nucleotides EF-1α promoter: bases 386-1545 T7 promoter/priming site: bases 1546-1565 Multiple cloning site: bases 1591-1681 myc … friday the 13th bgghttp://www.biovector.net/product/44170.html friday the 13th best to worstWebRK2载体价格3800元，RK2载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄、卡那、四环素，质粒大小为56 kb。RK2载体是质粒。 fat patties food truck ncWebOct 29, 2024 · The total amount of DNA was adjusted with empty vector pEF6/Myc-His. After 48 h, cell lysates were prepared and immunoprecipitated by anti-FLAG antibody resin. Each sample was analyzed by western blotting with the indicated antibodies. 3.2. Depletion of DCAF7 or TCP1α reduces the cellular level of ERCC1-XPF fat pats in lake charles laWebAddgene inc pef6 myc his Pef6 Myc His, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, … fat patties beaufort sc menuWebFeb 22, 2024 · Chinese hamster ovary cells were transiently transfected with the recombinant pEF6/Myc-His vector containing the core/E1/E2 insert. Transfection was performed by 2 electroporation shocks at... friday the 13th bikersThe control plasmid, pEF6/Myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice. Experimental Outline Use the following outline to clone and express your gene of interest using pEF6/Myc-His. 1. Consult the multiple cloning sites described on pages 3-5 to determine friday the 13th birthday shirt