Phloretin hydrolase
WebbPhloroglucinol is an organic compound with the formula C 6 H 3 (OH) 3.It is a colorless solid. It is used in the synthesis of pharmaceuticals and explosives.Phloroglucinol is one of three isomeric benzenetriols.The other two isomers are hydroxyquinol (1,2,4-benzenetriol) and pyrogallol (1,2,3-benzenetriol). Phloroglucinol, and its benzenetriol isomers, are still … Webb1 okt. 2004 · The phloretin hydrolase was heterologously expressed in Escherichia coli and purified. The molecular mass of the native enzyme was approximately 55 kDa as …
Phloretin hydrolase
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Webb1 okt. 2004 · Phloretin hydrolase was assayed in 50 mM potassium phosphate buffer (pH 7.0) containing 60 μM phloretin (Roth, Karlsruhe, Germany) added from a 1.2 mM stock solution in dimethyl sulfoxide (DMSO). DMSO (final concentration, 5% [vol/vol]) reduced the phloretin hydrolase activity by approximately 15%. Webb2,4-DAPG; Friedel–Crafts hydrolase; MAPG; PhlG; phloretin hydrolase Correspondence Y.-X. He, MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China Tel: +86 0932 8912563 E-mail: [email protected] Jian-Ting Han and Si-Ping Zhang contributed equally to this work
WebbIn enzymology, a phloretin hydrolase (EC 3.7.1.4) is an enzyme that catalyzes the chemical reaction. phloretin + H 2 O phloretate + phloroglucinol. Thus, the two substrates of this enzyme are phloretin and H 2 O, whereas its two products are … Webb19 feb. 2024 · The phloretin hydrolase ErPhlG was the first characterized PhlG homologous protein through gene library based biochemical screening . Based on this …
Webb1 apr. 2024 · The family of PhlG proteins catalyses the hydrolysis of carbon-carbon bonds and is widely distributed across diverse bacterial species. Two members of the PhlG … Webb1 feb. 2024 · Phloretin hydrolysis as carried out by phloretin hydrolase [25, 26] and the three-step conversion of daidzein to equol [27][28][29] are relatively well studied, while gut bacterial enzymes ...
WebbThe phloridzin’s poor oral bioavailability is primarily attributed to the presence of lactase-phloridzin hydrolase in the microvillar membrane of the small intestine. 12 This enzyme, intended for the hydrolysis of lactose, hydrolyses the β-glycosidic bond of phloridzin, resulting in the formation of phloretin and glucose.
Webbphloretin hydrolase Alternative Name(s) C-acylphenol hydrolase: Reaction catalysed; H2O + phloretin => 1,3,5-trihydroxybenzene + H(+) + phloretate: Comment(s) Also hydrolyzes … jamie sadock ladies golf clothesWebb5 juli 2004 · UniProt website fallback message If you are not seeing anything on this page, it might be for multiple reasons: You might have JavaScript disabled: make sure to ... lowest character value cosmosWebb1 mars 2014 · Phloretin hydrolase catalyzes the hydrolytic C-C cleavage of phloretin to phloroglucinol and 3-(4-hydroxyphenyl)propionic acid during flavonoid degradation in Eubacterium ramulus. lowest character evernoteWebbPhlorizin is a glucoside of phloretin, a dihydrochalcone. A white solid, samples often appear yellow owing to impurities. It is of sweet taste and contains four molecules of water in the crystal. Above 200 °C, it decomposes to give rufin. It is poorly soluble in ether and cold water, but soluble in ethanol and hot water. jamie rugers firearm shopWebbLactase-Phlorizin Hydrolase. A multifunctional protein that contains two enzyme domains. The first domain (EC 3.2.1.62) hydrolyzes glycosyl-N-acylsphingosine to a sugar and N … lowest character in ascii codeWebbIn enzymology, a phloretin hydrolase(EC3.7.1.4) is an enzymethat catalyzesthe chemical reaction The Enzyme Commission numberis a numerical classification scheme for … lowest character pythonWebbThe enzyme phloretin hydrolase was purified from cells of E. herbicola Y46 grown on Yeast Beef Broth, by treatment of crude extracts with protamine sulfate, ammonium … jamie runyon probation officer