Photocleavable spacer

WebPhotocleavable spacer modified oligonucleotides could be used to tether an antisense oligo to a dextran, which is too large to pass through the nuclear pores. Photocleavage would … WebJan 6, 2003 · This unique property of the photocleavable (PC) linker has promoted its application as a tool for separating, purifying, and identifying desired target biomolecules (6–8). The PC spacer phosphoramidite provides a potentially convenient tool for attachment of a wide range of functional groups to DNA by using solid-phase synthesis chemistry ( 8 ...

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WebThe light-triggered release of paclitaxel or fluorophore Cy5 was achieved by incorporation of photocleavable spacers into the RNA nanoparticles. Upon irradiation with ultraviolet light, cargos were rapidly released (within 5 min). In vitro treatment of breast cancer cells with the RNA nanoparticles harboring photocleavable paclitaxel showed ... WebPhotocleavable spacer modified oligonucleotides could be used to tether an antisense oligo to a dextran, which is too large to pass through the nuclear pores. Photocleavage would then be used to release the oligo, thereby decoupling antisense effects within the cytoplasm from antisense effects within the nucleus. how to remove minerals from tap water at home https://johnogah.com

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WebMar 19, 2003 · New cationic lipids having an o-nitrobenzyl moiety as a photocleavable spacer between its hydrophilic and hydrophobic region were synthesized. To improve the efficiency of transfection with lipoplexes, … WebBio-Synthesis offers photocleavable PC Biotin Oligo Modification at various scales. This light activatable PC biotin modified oligonucleotide contained a photocleavable spacer between the biotin group and the DNA bases. The biotin group is separated from the 5' end nucleotide base by the photo-cleavable group and a long chain spacer arm. Webunique property of the photocleavable (PC) linker has promoted its application as a tool for separating, purifying, and identifying desired target biomolecules (6–8). The PC spacer phosphora-midite provides a potentially convenient tool for attachment of a wide range of functional groups to DNA by using solid-phase synthesis chemistry (8, 9). norgraf antofagasta

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Photocleavable spacer

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WebJan 1, 2024 · Applying a photocleavable linker in drug conjugation serves as an effective strategy for actively controlling such payload release using a light stimulus. This chapter … WebPhotocleavable Modifications. Explore our photocleavable analogues of the Amino- and Spacer-Modifiers and the Biotin label, as well as our PC linker molecule. The general design of the PC monomers is based on an α-substituted 2-nitrobenzyl group. The photo-reactive group is derivatised as a cyanoethyl phosphoramidite that can be incorporated ...

Photocleavable spacer

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WebPhotocleavable PC linker contained a non-nucleosidic group that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm. Upon … WebStorage Conditions: -20°C To -70°C. Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free …

WebPhotocleavable DBCO-NHS ester reagent contains an amine reactive group linked to DBCO moiety through a spacer arm containing a photocleavable moiety. Molecules jointed by the copper-free click reaction can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW ... WebThe spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer …

WebSpacer Modifiers. The hydrophilic spacers; Spacer 9 ( LK2128) and Spacer 18 (HEG) ( LK2129) are also particularly useful to build in flexibility within an oligo sequence. 6. As with 3’-‘spacers’ these are known to be nuclease … http://www.rx-8.com/chanpinzhongxin/210204.html

WebPC Spacer is a photocleavable C3 spacer modifier. Our ability. We provide a flexible ordering model to quickly meet your order requirements, whether it is a large number of standard products or customized products. With a GLP laboratory and unique technology development, we can help you meet the ever-changing market needs. ...

WebThe PC spacer (LK2131) and PC linker (LK2066) amidites can be incorporated within an oligonucleotide sequence. This is particularly useful in adding a modifier as a phosphoramidite where there is a need to remove this from the oligonucleotide at a later stage. ... Photocleavable Peptide-Oligonucleotide Conjugates for Protein Kinase Assays … norgren auto drain leakingWebConveniently introduce spacer modifications into your oligonucleotide during synthesis with our high-quality spacer phosphoramidites and solid supports. Adding a spacer adds … norgren air cylinder distributorWebPC Biotin Alkyne an azide-reactive photocleavable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to an azide group through a spacer arm containing a photocleavable moiety. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 ... how to remove mineral oilWebPC Spacer (photocleavable) is a non-nucleosidic molety that can be used as an intermediary to attach any available phosphoramidite modification at either end of an oligonucleotide … norgran recordsWebPC Linker (photocleavable) Spacer C3. Spacer C12. Spacer 9 is a triethylene glycol chain that is 9 atoms long (6 carbons + 3 oxygens), and is used to incorporate a spacer arm into an oligonucleotide. Spacer 9 can be incorporated in consecutive additions whenever a longer spacer is required. Spacer 9 has been used to form non-nucleotide bridges ... norgren country of originWebAug 1, 1995 · This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any … norgine walesnorgran f74 replacement